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Abstract
Objective: To evaluate the effect of the synthetic C4-HSL and 3OC12-HSL molecules produced by wild strains of P. aeruginosa as promoters of the root system of Solanum lycopersicum L in vitro.
Design/methodology/approach: Wild and control strains were studied, in which crude extraction in ethyl acetate and quantification of both molecules were performed. Different concentrations of synthetic 3-oxo-C12-HSL and C4-HSL (10, 12, 24, 48, 96, and 192 μM) were used. The ability to elongate the primary root, as well as the length and density of root hairs under conditions were evaluated. Laboratory-controlled studies.
Results: The results obtained were that the crude extracts showed no beneficial effect on PAO1, and that the double mutant in the genes encoding the synthases had the same effect as the positive control, confirming that the effect was not due to the presence or absence of these AHLs. However, when the synthetic molecules 3-oxo-C12-HSL and C4-HSL were evaluated, the in vitro effect on the primary root at 24 µM and root hair density at 96 µM concentrations, respectively, was significant (p < 0.05) compared to the controls.
Findings/conclusions: In this work, we detected that the concentration of C4-HSL and 3OC12-HSL molecules in S. lycopersicum showed in vitro effects on the primary root at 24µM and root hair density at concentrations of 96µM respectively, and that the concentrations extracted in the crude extract with ethyl acetate are unable to generate a positive effect.